Cytotrophoblasts (CTBPRO2™)
Cytotrophoblasts are progenitors that can give rise to mature trophoblasts responsible for implantation and maintenance of the maternal-fetal barrier throughout gestation.
- Isolated from full term placenta after live birth, posing no risk to mother or child
- Can be expanded through 10 population doublings when cultured as directed
- Successful expansion requires CTBPRO2GRO™ Media, Collagen-IV culture surface, and a low oxygen culture environment
- Cells are immunopositive for CD49f, EGFR, and CK7 and can be induced to secrete human chorionic gonadotropin (hCG)
PRICE:
$1,350.00 – $1,500.00
Cytotrophoblasts are the fetal-origin epithelial cells that partner with the placental and fetal microvascular endothelial cells for creating a barrier between the maternal blood and the developing baby. The cytotrophoblasts act as lineage-restricted progenitor cells which can self-renew and give rise to two different types of trophoblasts. Extravillous trophoblasts are the highly migratory cells responsible for invading the uterine wall early in gestation and ensuring proper implantation of the fertilized egg. Syncytiotrophoblasts arise from cytotrophoblasts and form a contiguous layer of multinucleated cells that are tightly interconnected to control passage of nutrients, waste, toxicants, and pathogens between the mother and developing baby.
Until recently, it has been challenging to conduct work in vitro with cytotrophoblasts or their derivatives due to lack of primary cells. Surrogates have included choriocarcinoma-derived cells and genetically modified immortalized cells, both of which may lack physiologically relevant features present in primary human cells. While cytotrophoblasts have been isolated and propagated successfully from pre-term, early gestation placentae, many labs opt out of using these due to ethical concerns and/or restrictions on the use of pre-term tissue. In the past, in vitro experiments have necessitated the use of primary, freshly isolated, senescent trophoblasts that have less than a 24 hour usage.
The Amnion Foundation has developed methods that enable the isolation and propagation of cytotrophoblast progenitor cells from full term placenta, thereby creating a non-controversial resource for these valuable cells. Through tight control of tissue procurement, isolation protocols, and the culture environment, term cytotrophoblasts can be isolated, propagated and cryopreserved to enable their use in genomic studies and cell-based assays.
- Derived from human term placenta after a healthy birth and are fetal in origin
- 500K cells / vial post-thaw
- Product Code #
- AF1230 – Price: $1,350.00
- Single donor origin (non-pooled)
- Multiple lots available from independent donors
- Passage Level: P-3 to P-4
- Phenotypically characterized by flow cytometry
- Cells can be serially passaged through at least 10 additional population doublings from time of thaw
- Confirmed non-reactive for HBsAg, HBCore, HIV-1/HIV-2 + O, HTLV I/II, HIV/HCV/HBV NAT, West Nile Virus NAT
- Sterile; screened for endotoxin
- This non-GMP product is for research use only and cannot be used in therapy or diagnostics.
- Certificate of Analysis
- Instructions for Use
- Storage: < -150°C (Liquid Nitrogen)
- Shipped On: Dry Ice or Cryoport (customer preference)
Cytotrophoblasts (CTBPRO2™)
